Transitional Analysis - Chromatography Column Viability

The overall health of your chromatography column is key to providing proper protein separation in your

downstream purification operations.  While salt spike injections can provide some statistical parameters

like HETP* and Asymmetry values, determining these values sometimes comes at a price - additional

cleaning, increased cycle times, decreased throughput.

And how are you computing your statistical parameters?  Believe it or not, some folks still print out a

time-based trace of their analyzer signal, eyeball a peak and measure things with a ruler.

How about timeliness?  There's real-time, near real-time, and offline.  And if you're printing off a trend

or exporting time-based data to Excel and sneaker-netting it over to another system, that's not real-

time.

Transitional Analysis is based on evaluating the analyzer curve produced by transitioning from one

buffer to another.  Since this kind of transition occurs during normal column sequencing, no additional

processing steps or lost production is encountered.

We have developed a real-time Transitional Analysis package that produces the statistical parameters

you need to monitor the health and well-being of your chromatography column.  Based on Emerson's

DeltaV automation platform, our solution can be integrated into any DCS or PLC system, laboratory

to 1000 liter columns and beyond.

Key Features:

• 9 Statistical output parameters including HETP, Skewness, and Standard Deviation

• Volume based data collection to normalize flow disruptions and repack volume changes

• Results available within 30 seconds of transition completion

• Optional offline tool allows processing of historical data using the same online logic

For more information, please visit TransitionalAnalysis.net

or contact Bruce Greenwald at 704-375-4465 ext 253